p70 s6 kinase  (Cell Signaling Technology Inc)

Journal: The Journal of Physiology

Article Title: Muscle regeneration is improved by hot water immersion but unchanged by cold following a simulated musculoskeletal injury in humans

doi: 10.1113/jp287777

Figure Lengend Snippet: Figure 9. Representative blots and quantification of phosphorylated mTOR (A, B), phosphorylated-S6 (C, D) and phosphorylated-p70 (E, F) protein expression at PRE, D5 and D11 in CWI, TWI and HWI groups Data are presented as medians along with individual data. #Significantly different from PRE (P < 0.05); ##significantly different from PRE (P < 0.01); †significantly different from D5 (P < 0.05); ∗significantly different between groups (P < 0.05).

Article Snippet: The membranes were incubated overnight at 4°C with the following primary antibodies: rabbit polyclonal phosphorylated nuclear factor-κB (NF-κB) p65 (1:1000, cat. no. 3033, Cell Signaling Technology, Danvers, MA, USA), monoclonal rat interleukin (IL)-10 (1:500, sc-73309, Santa Cruz Biotechnology, Dallas, TX, USA), mouse monoclonal HSP70 (1:750, ADI-SPA-810, Enzo, Farmingdale, NY, USA), mouse monoclonal HSP27 (1:750, ADI-SPA-800, Enzo Life Sciences, Farmingdale, NY, USA), rabbit polyclonal phosphorylated mammalian target of rapamycin (mTOR; 1:1000, cat. no. 2971, Cell Signaling Technology), rabbit polyclonal phosphorylated S6 ribosomal protein (1:1000, cat. no. 5364, Cell Signaling Technology), mousemonoclonal p-p70 S6 kinase (1:1000, cat. no. 9206, Cell Signaling Technology) rabbit polyclonal vascular endothelial growth factor (VEGF; 1:500, sc507, Santa Cruz Biotechnology) and rabbit polyclonal transforming growth factor β (TGF-β 1:1000, cat. no. 3711, Cell Signaling Technology, Danvers, MA, USA).

Techniques: Expressing